Antimicrobial and Anti-inflammation Activities of Fraction and Single Peptides Derived from Mare Milk Protein

Abstract

Mare milk protein contains bioactive peptide which is beneficial for human and animal health. Peptides in the fraction and single may show different activities. The objectives of the study were to evaluate antimicrobial and anti-inflammation activities of the fraction and single peptide derived from mare milk protein. Antimicrobial assay was conducted by testing antibacterial and antifungal activities of fraction <3 kDa, single peptide LVNELTEFAK (peptide 1), HPYFYAPELLYYANK (peptide 2), and LANSLTEFAK (peptide 3) against Escherichia coli and Candida albicans. Antioxidant assay was conducted using 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. Anti-inflammation effect was detected by interleukin 1-β (IL-1β) and Tumor Necrosis Factor-α (TNF-α) production in mice after administration of Escherichia coli’s lipopolysaccharide (LPS) and combined with fraction or single peptide. Result showed that peptide in fraction form has higher antibacterial, antifungal activities and antioxidant activities against radical ABTS than all of single peptide (P<0.05). Anti-inflammation activity was showed by peptide 1 and peptide 2 which was indicated by significantly decreasing of IL-1β after treatment (P<0.05). Based on the results, it was concluded that antimicrobial and anti-antioxidant activities fraction <3 kDa was better than single peptide. For anti-inflammation activity, a single peptide showed better activity than fraction.