Abstract
The Escherichia coli strain JA221 can suppress the UAG stop codon, although the existence of an amber suppressor tRNA has not previously been described for this strain. When using a plasmid to express α‐sarcin, which has TAG as its stop signal, two proteins were obtained: a smaller protein corresponding in size to that of the expected protein, and a larger protein, which could be accounted for by the presence of a second stop codon (TGA) 18 base pairs downstream of the original. This feature of strain JA221 must therefore be considered when using this strain as a host for the production of recombinant proteins. Copyright © 1995, Wiley Blackwell. All rights reserved
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CITATION STYLE
Lacadena, J., del Pozo, A. M., Mancheño, J. M., Gasset, M., Oñaderra, M., & Gavilanes, J. G. (1995). Escherichia coli JA221 can suppress the UAG stop signal. Letters in Applied Microbiology, 21(2), 96–98. https://doi.org/10.1111/j.1472-765X.1995.tb01015.x
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