Pear in Vitro Propagation Using a Double-phase Culture System

Abstract

Proliferation of Pyrus communis L. cv . Abate Fetel, Precoce Morettini, and Guyot was accomplished with a yield of 10 to 15 new shoots per explant. The in vitro procedure is based on the use of 6.7 μ m BAP as an overlay on a modified MS medium. Rooting without callus formation was achieved by immersing the basal end in 5 μ m IBA solution for 1 min. The possible inhibition of proliferation and plantlet regeneration by GA 3 and IBA is discussed. Chemical names used: 6-benzylaminopurine (BAP); indole-3-butyric acid (IBA); gibberellic acid (GA 3 ).