Synthesis of cross-linked triple helical DNAs possessing high affinity to triple helical DNA binding protein.

Abstract

We examined binding ability of MBP-LOR3(ARF) protein to triplexes composed of branched oligonucleotides possessing a pentaerithritol linker. It was found that the triplex, which has an antraquinonecarbonyl group at the 5'-end of the third strand and is connected with the pentaerithritol linker, has greater affinity to the MBP-LOR3(ARF) protein than an unmodified triplex.