Identificação do potencial amilolítico de linhagens mutantes do fungo filamentoso Aspergillus nidulans

Abstract

The amylases are among the most important industrial enzymes showing great biotechnological importance, mainly in the food industry. Due to the advance knowledge in enzyme research, the use of fungi as source of enzymes has acquired a prominent status in most varied industrial and commercial areas. Thus, the present study tried to identify the presence of the amylolytic activity in four stains of filamentous fungi Aspergillus nidulans, selvagem, PAT, biA1methG1 and CLB3 by using two different culture means, PDA and Complete mean with 2% starch, varying the treatments with and without the addition of glucose. The colony average diameter, the halo average diameter, and the enzymatic index were determined. The results show that all strains tested were capable of degrading starch in the absence of glucose although the treatment that presented statistically better growing and bigger degradation of starch was CM without glucose with 2% starch and the strain that demonstrated potentially degradation was CLB3 mutant. It was concluded that Aspergillus nidulans can be considered as an enzyme producer.