Comparative aminoglycoside inactivation by β-lactam antibiotics effect of a cephalosporin and six penicillins on five aminoglycosides

Abstract

Gentamicin, tobramycin, netilmicin, kanamycin and amikacin were evaluated over time for biologic activity in human serum, in combination with 6 β-lactams. Simple addition of aminoglycoside and 250 μg/ml penicillin produced aminoglycoside inactivation at 8 ~ 48 hours. However, all β-lactam antibiotics exhibited decay in human serum at 37°C, even when present as a single component. All aminoglycosides could be inactivated by penicillins but differed markedly in their susceptibility. Amikacin, at 20 μg/ml, was the least inactivated by any penicillin; netilmicin, at 10 μg/ml, was the next least inactivated. Tobramycin had pronounced loss of biological activity exceeding that of any aminoglycoside, appearing as early as 8 hours. The ability of the various penicillins to produce aminoglycoside inactivation, in approximate descending order, was; carbenicillin, ticarcillin, penicillin G, oxacillin, methicillin, ampicillin. Cephalothin produced minimal inactivation. Aminoglycoside inactivation also occurred at 25°C, and with many samples stored at 4°C, although at proportionately slower rates. For samples stored at — 20°C, only tobramycin had substantial loss of activity. These data indicate that adequate handling and prompt assay of the specimen are important. © 1982, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.