A new liquid medium without blood and serum for culture of hemoflagellates

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Abstract

A liquid medium without blood or serum was developed for cultivation of hemoflagellates. To a basic LIT medium containing liver infusion broth and tryptose, a mixture of RPMI 1640 and Medium 199 was added. This combination permitted high parasite yields useful for biochemical and immunological studies.

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Sadigursky, M., & Brodskyn, C. I. (1986). A new liquid medium without blood and serum for culture of hemoflagellates. American Journal of Tropical Medicine and Hygiene, 35(5), 942–944. https://doi.org/10.4269/ajtmh.1986.35.942

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