Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells

Abstract

Purpose The purpose of this study was to identify agerelated oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates. Methods We used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism. Results CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In goodquality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos. Conclusions These potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes. © Springer Science+Business Media, LLC 2010.