Analytical performance of the various acquisition modes in Orbitrap MS and MS/MS

Abstract

Abstract The quadrupole Orbitrap mass spectrometer has revolutionized high‐end analysis because it delivers high‐resolution full scan acquisitions that can be combined with additional acquisition modes where the quadrupole isolates a particular mass range prior to fragmentation and HRMS‐based acquisition. Depending on the user's specific requirements for sensitivity, selectivity, linear dynamic range, and speed of analysis, the performance characteristics of these different acquisition modes need to be considered and the most appropriate approach adopted with the overarching objective of the analysis in mind. In this Special Feature, with a focus on multi‐residue analysis, Dr. Anton Kaufmann discusses the operation of the quadrupole Orbitrap mass spectrometer to meet specific detection and quantification requirements in the analysis of targeted compounds. He describes and discusses the capability and limitations of the current Q Orbitrap scan modes, the expected selectivity and sensitivity performance, the need to maintain sufficient data points across a chromatographic peak, and additional requirements relating to quantification. To help the user understand the underlying technical measurement principles, Dr. Kaufmann also provides a set of informative diagrams that illustrate the features of the different scan modes.