Comparative evaluation of antibacterial effect of nanoparticles and lasers against Endodontic Microbiota: An in vitro study

Abstract

Background: Present study was conducted with the aim of evaluating antimicrobial efficacy of silver (AgNP) and gold nanoparticles (AuNP) with and without Nd: YAG laser (L) irradiation against experimentally inoculated Enterococcus faecalis in infected human root dentin. Material and Methods: 120 extracted single rooted human teeth were prepared and inoculated with E. faecalis for 24 hrs. The teeth were then randomly divided into 4 experimental group: AgNPs group: irrigation for 3minutes with 50 µl of 100 ppm, the AuNPs group: irrigation with 50 µl of 100 ppm, the AgNPs & Nd: YAG lasers group: irrigation with 50 µl of 100 ppm + irradiation with 1.5W laser for 60 seconds, the AuNPs & Nd: YAG lasers group: irrigation with 50 µl of 100 ppm + irradiation with 1.5W laser for 60 seconds. One control group consisting of 2% CHX irrigation for 3 minutes was also there (n = 20). The specimens were collected from the canal before and after irrigation, and colony forming units were observed. Results: significant difference was found among all the groups in comparison to the control group (p<0.05). The greatest reduction in CFU's was observed with combination of AgNPs & Nd: YAG lasers group. Conclusion: AgNPs in combination with Nd: YAG laser irradiation has the potential to be used as root canal disinfectant.