Création de variétés tolérantes au stress abiotique chez l'orge (Hordeum vulgare L.) par culture d'anthères

Abstract

The study aims the creation of the pure barley variety, tolerant to the abiotic stress by anther culture. This study was concerned two variety of barley: cultivar Ardhaoui, selected for their best tolerance to the abiotic stress (salt and drought) collected from Tunisia, and cultivar Arige, from Syria, sensitive for the two types of stress. The androgenic aptitude was evaluated in the parents (Ardhaoui and Arige) and their hybrids (F1) by anther culture. The stage of setting in anther culture was given on the basis of morphological and cytological criteria. The result shows that the capacity of regeneration of cal differs from a genotype with another (it varies from 50% at the hybrids with 40% in the parent Arige). Following the results, it is important to announce that the rate of albinism which we observed is height, it especially exceeds 70% from Ardhaoui; it can be explained by the combination of several factors. Finally we can deduced that the majority of the regenerated planted are diploid (70%). © 2010, Taylor & Francis Group, LLC.