Basic Investigation for Classification of Anticancer Drugs by Pharmacological Effects

Abstract

777 a 北海道大学大学院医学研究科， b 同院薬学研究院臨床 薬剤学研究室， c 同薬物動態学研究室， d 北海道大学病 院薬剤部 現所属： † 弘前大学医学部附属病院薬剤部， † † 神戸大学 医学部附属病院薬剤部  The most eŠective drugs based on the type of cancer are chosen for chemotherapy. Tumor cells can be targeted at the DNA, RNA or protein level, and most of the classical anticancer drugs interact with tumor DNA in a time-dependent manner or a concentration-dependent manner. However, it has been unclear to date whether a combination therapy is carried out by using exact classiˆcation. Thus it is necessary to reclassify a great number of anticancer drugs. We propose a new classiˆcation system based on pharmacological eŠects of anticancer drugs. Classiˆcation of four anticancer drugs (cisplatin, carboplatin, paclitaxel and gemcitabine) was performed by the 3-(4,5-Dimethyl 2-thiazolyl) 2,5-diphenyl 2H-tetrazolium bromide (MTT) assay. The four anticancer drugs were grouped by IC 50 values (inhibitory concentration, 50％) in a time-dependent manner and a concentration-dependent manner. The present approach may be combined to enhance the chemosensitivity, improve the dose of cytotoxic drugs and evaluate the eŠects of novel antican-cer drugs.