The organization of the Golgi complex and microtubules in skeletal muscle is fiber type-dependent

Abstract

Skeletal muscle has a nonconventional Golgi complex (GO), the organization of which has been a subject of controversy in the past. We have now examined the distribution of the GC by immunofluorescence and immunogold electron microscopy in whole fibers from different rat muscles, both innervated and experimentally denervated. The total number of GO elements, small polarized stacks of cisternae, is quite similar in all fibers, but their intracellular distribution is fiber type-dependent. Thus, in slow- twitch, type I fibers, ~75% of all GO elements are located within 1 μm from the plasma membrane, and each nucleus is surrounded by a belt of GO elements. In contrast, in the fast-twitch type IIb fibers, most GC elements are in the fiber core, and most nuclei only have GC elements at their poles. Intermediate, type IIA fibers also have an intermediate distribution of GO elements. Interestingly, the distribution of microtubules, with which GC elements colocalize, is fiber type-dependent as well. At the neuromuscular junction, the distribution of GC elements and microtubules is independent of fiber type, and junctional nuclei are surrounded by GC elements in all fibers. After denervation of the hindlimb muscles, GO elements as well as microtubules converge toward a common pattern, that of the slow-twitch fibers, in all fibers. Our data suggest that innervation regulates the distribution of microtubules, which in turn organize the Golgi complex according to muscle fiber type.