Preliminary evaluation of the immunoenhancement potential of fowl typhoid vaccine formulated as an oral cationic liposome

Abstract

Salmonella gallinarum is a non-motile host-specific bacterium in domestic poultry. It causes fowl typhoid, especially in domestic poultry. The study was performed with a view to compare the antibody titre of commercial subcutaneous Fowl typhoidReg. vaccine with its oral liposomal formulation. The chicken, divided into three groups (n=20 per group), were inoculated via oral or subcutaneous route. Group A was given commercial subcutaneous Fowl typhoidReg. vaccine, Group B served as the unvaccinated control and Group C was given the Fowl typhoidReg. vaccine encapsulated in a cationic liposome. Vaccination of the groups was carried out once at 42 days of age. Blood samples were collected from the jugular vein at 56 days of age to obtain the sera for slide and microplate agglutination test. At 63 days of age, the birds were challenged with a field strain of the S. gallinarum. From the microplate agglutination test (mean+or-standard deviation (SD), at p<0.05), it was found that there was no significant difference in the mean antibody titre of the birds by either the subcutaneous or oral vaccination of Fowl typhoidReg. vaccine.