Pure Culture Synthesis of Douglas-Fir Mycorrhizae with Species of Hebeloma, Suillus, Rhizopogon, and Astraeus

Abstract

Four species of fungi formed mycorrhizae with Pseudotsuga menziesii (Mirb.) Franco roots in a new, simple apparatus that permits seedling tops to grow in the open while roots grow in pure culture with an inoculated fungal isolate. Morphology and anatomy of mycorrhizae formed with eachfungus are described in detail. The need for preserving voucher specimens of fungi reported as mycorrhizal associates is emphasized. Detailed descriptions of mycorrhizae formed by known fungi on specified hosts are needed as a basis for mycorrhiza classification.