A validated method using RP-HPLC For quantification of reserpine in the Brazilian Tree Rauvolfia sellowii Müll. Arg. (Apocynaceae)

Abstract

This study describes a simple, fast and reproducible method using RP-HPLC-UV, in a gradient system, for quantification of reserpine in Rauvolfia sellowii stem bark. The analysis were carried out on a C18 column; mobile phase was water and acetonitrile, and separations were carried out in 10 min, flow rate of 1.0 mL min -1, 25 °C and 268 nm. The validation data showed that the method was specific, accurate, precise and robust. Results were linear over a range of 0.625-40.0 mg mL -1, and the mean recovery was 95.1%. The amount of reserpine found in the dried stem bark was 0.01% (m/m).