The neonatal Fc receptor is expressed by human lymphocytes

Abstract

Methods Freshly isolated PBMCs were obtained from three healthy volunteers and one buffycoat. NK, B, monocyte and CD4 + and CD8+ cell subsets were sorted by flow cytometry. FcRn and beta-2-microglobulin (b2M) mRNA levels were determined by RQ-PCR. FcRn protein expression from PBMCs obtained from a buffycoat was analyzed by Western blot studies. Author details 1Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands. 2Department of Immunology, Erasmus University Medical Center, Rotterdam, The Netherlands. 3Rotterdam Eye Hospital, Rotterdam, The Netherlands. Published: 25 November 2010 References 1. Roopenian DC, Akilesh S: FcRn: the neonatal Fc receptor comes of age. Nat Rev Immunol 2007, 7(9):715-25. 2. Zhu X, Meng G, Dickinson BL, Li X, Mizoguchi E, Miao L, Wang Y, Robert C, Wu B, Smith PD, Lencer WI, Blumberg RS: MHC class I-related neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal macrophages, and dendritic cells. J Immunol. 2001, 166(5):3266-76. doi:10.1186/1479-5876-8-S1-P1 Cite this article as: van Bilsen et al.: The neonatal Fc receptor is expressed by human lymphocytes. Journal of Translational Medicine 2010 8(Suppl 1):P1. Results RQ-PCR revealed expression of FcRn and B2M mRNA in B, CD4+ and CD8+ cells and monocytes in all three RPE cultures. Western blot analysis demonstrated that mRNA expression in PBMCs from a buffycoat co-existed with FcRn protein expression. Conclusions Apart from monocytes, human B, CD4+ and CD8+ cells express low levels of FcRn.