Simulated Lymphatic Fluid for In-Vitro Assessment in Pharmaceutical Development

Abstract

In addition to removing excess extracellular fluid and mobilizing immune cells throughout the body, lymphatic fluid provides a means for drug transport. Lymphatic drug delivery can impart higher efficacy and bioavailability, especially following oral administration. Currently, there is no standardized composition for simulated lymphatic fluid. Standardization of a simulated lymphatic fluid media would be an important and novel contribution to fill the current void in this emerging area of drug and formulation development. This study aims to review and analyze the composition and flow rate of prepared simulated lymphatic fluid with comparison to commercially available artificial fluid and biological fluid. The prepared simulated fluids were closer in makeup to biological fluid than the commercial artificial fluid, which suggests potential benefits for developing and optimizing lymphotropic formulations for in vitro studies.