Acción antimicrobiana in vitro de dentífricos conteniendo fitoterápicos

Abstract

The aim of this study was to evaluate and compare in vitro antimicrobial potential of 7 dentifrices containing phytotherapics agents oral bacteria obtained from saliva and standard strains of S. mutans ATCC 25175, S. sanguis ATCC 10556, and L. casei ATCC 4646. For this purpose, concentrated test and control solutions were obtained by mixing 3 grams of each in 10 ml of sterile deionized water which was submitted to centrifugation, the sublimate obtained was further dissolved in a ratio of 1:2 to 1:32. A diffusion test in agar was carried out by sowing the standard strain and stimulated total saliva. Disks soaked in a dentifrices solution and then placed on culture disks were then incubated in anarobiosis for 48 hours. Inhibition halos were then measured. Results obtained from ANOVA statistical analysis. However, comparison in the presence of a positive control, revealed that against standard strains, the pure solution of the dentifrices had antimicrobial activity, with the exception of Gessy Cristal®. Against oral bacteria recovered from saliva, diluted dentifrices to 1:2 showed the same activity, excepting Parodontax®.