Hydrogen peroxide is involved in cGMP modulating the lateral root development of Arabidopsis thaliana

Abstract

3',5'-cyclic guanosine monophosphate (cGMP) and hydrogen peroxide (H2O2) function as the important signaling molecule which promote the lateral root development of Arabidopsis thaliana. In this study, interestingly, application of 8-Br-cGMP (the membrane permeable cGMP analog) promoted the endogenous H2O2 production. In addition, the decrease of endogenous H2O2 also inhibited the effect of cGMP on the lateral root development. Thus, H2O2 maybe act as a downstream signaling of cGMP molecule which is involved in the lateral root development of Arabidopsis. We further found that H2O2 affected cGMP modulating polar auxin transport. When the endogenous H2O2 level was inhibited, the effect of cGMP on the acropetal auxin transport and the basipetal auxin transport was removed. Moreover, pin2 was insensitive for cGMP and H2O2 suggesting that PIN 2 protein plays an important role in cGMP and H2O2 modulating the lateral root development of Arabidopsis. © 2013 Landes Bioscience.