Use of chitosan gel for the purification of protein

Abstract

The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 μm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.