Catechol Biosensor Design Based on Ferrocene-Derivatized 2,5-Dithienyl Pyrrole Copolymer with 3,4-Ethylenedioxythiophene

Abstract

Novel conducting platforms based on co(polymerization) of 2,5-dithienyl pyrrole-ferrocene and 3,4-ethylenedioxythiophene were employed for the first time in developing a catechol biosensor. The grafting of Fc moiety onto a hybrid thienyl pyrrole monomer for phenolics detection is proposed for the first time herein to enhance the efficiency of electrochemical reduction of quinone and, in turn, improve the stability of the biosensor in a ‘reagentless’ manner. Tyrosinase enzyme was immobilized by cross-linking onto the carbon nanotubes-enriched electrodeposited films, and catechol was determined with a low detection limit of 2.1 µM. Good operational stability (RSD 3.34 %) was observed during 20 consecutive measurements.