Immunization with recombinant enterovirus 71 viral capsid protein 1 fragment stimulated antibody responses in hamsters

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Abstract

Enterovirus 71 (EV71) causes severe neurological diseases resulting in high mortality in young children worldwide.Development of an effective vaccine against EV71 infection is hampered by the lack of appropriate animal modelsfor efficacy testing of candidate vaccines. Previously, we have successfully tested the immunogenicity andprotectiveness of a candidate EV71 vaccine, containing recombinant Newcastle disease virus capsids that display anEV71 VP1 fragment (NPt-VP11-100) protein, in a mouse model of EV71 infection. A drawback of this system is itslimited window of EV71 susceptibility period, 2 weeks after birth, leading to restricted options in the evaluation ofoptimal dosing regimens. To address this issue, we have assessed the NPt-VP11-100 candidate vaccine in a hamstersystem, which offers a 4-week susceptibility period to EV71 infection. Results obtained showed that the NPt-VP11-100candidate vaccine stimulated excellent humoral immune response in the hamsters. Despite the high level ofantibody production, they failed to neutralize EV71 viruses or protect vaccinated hamsters in viral challenge studies.Nevertheless, these findings have contributed towards a better understanding of the NPt-VP11-100 recombinantprotein as a candidate vaccine in an alternative animal model system. © 2012 Ch'ng et al.; licensee BioMed Central Ltd.

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Chng, W. C., Stanbridge, E. J., Wong, K. T., Ong, K. C., Yusoff, K., & Shafee, N. (2012). Immunization with recombinant enterovirus 71 viral capsid protein 1 fragment stimulated antibody responses in hamsters. Virology Journal, 9. https://doi.org/10.1186/1743-422X-9-155

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