RFLP framework map using recombinant inbred lines in rice

Abstract

Recombinant inbred (RI) lines of rice (Oryza sativa L.) were developed from a cross of Asominori x IR24 by the single seed descent method. Seventy- one RI lines of the F6 and F7 generations were used for RFLP mapping. RFLP framework map constructed using the RI lines covered a distance of 1275cM containing 375 markers. All the twelve linkage groups were assigned to their respective chromosomes. The RI lines showed a distorted segregation in some regions of chromosomes 1, 3, 6, 11 and 12. Heterozygous loci in the lines still remained. Average frequency of the heterozygous region was 3.6% with a range of 0% - 19.3% in F6 and 1.9% with a range of 0% - 5.5% in F7. The RFLP map corresponded well to the previous maps constructed using F2 populations (Saito et al. 1991, Kurata et al. 1994) and the two previous maps were integrated in detail. The RFLP framework map and RI lines developed in this study will be useful for further accumulation of molecular markers and analysis of quantitative trait loci.