AlphaFold 3 accurately models natural variants of Helicobacter pylori catalase KatA

Abstract

Experimental structure determination is rarely performed for natural protein variants possessing only minor amino acid differences from published structures, even though small substitutions can significantly impact structure and function. Here, we present a case study showing that AlphaFold 3 can accurately model the structures of natural protein variants of catalase from Helicobacter pylori . However, providing an incorrect oligomeric state can reduce model accuracy—an error that non-expert users may easily make.