Optimization of culture conditions on the proliferation of aspergillus terreus n-gl1 strain isolated from curcuma longa L. By design-expert 6.0.6 and bc pharsoft software

Abstract

Curcuma longa L. is widely used as spices and medicines. Leaves, stems and rhizomes of the plant contain essential oils and provide a useful habitat for different endophytic fungi groups. Strain of Aspergillus terreus was isolated from Curcuma longa L. and was determined to be capable of producing secondary metabolites against bacteria by disc diffusion method and optimization of culture conditions by Design-Expert 6.0.6 and BC Pharsoft software. This research identified of the culture conditions for A. terreus N-GL1 strain produced secondary metabolites against Staphylococcus aureus and MRSA (Methicillin resistant Staphylococcus aureus) good: appropriate carbon source was saccharose 1% or molasses 2,2%; suitable nitrogen sources are potatoes 10% at pH 7; initial amount of yeast cells was 104 CFU/ml; incubated at room temperature for seven day. This study was found out as suitable conditions for A. terreus N-GL1 strain produced the secondary metabolites against S. aureus and MRSA.