Evaluation of in vitro cytotoxic activity of chloroform extract of Sida acuta Burm.F

Abstract

Objective: The aim of the present work was to investigate the in vitro cytotoxic activity of chloroform extract of Sida acuta Burm.f. Methods: The in vitro cytotoxic activity of chloroform extract was performed by 3-(4, 5–dimethyl thiazol–2–yl)–2, 5–diphenyl tetrazolium bromide assay method against A-431 (human epidermoid carcinoma) and HeLa (human cervical cancer) cell lines. Results: The various concentrations of crude chloroform extract (1000, 500, 250, 125, and 62.5 µg/ml concentration) of S. acuta whole plant were performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against A-431 cell lines (human epidermoid carcinoma) with an inhibit cell growth by 50% (IC50) of 375±0.00 and HeLa cell lines (human cervix carcinoma) with an IC50 of 610.00±2.5. The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the extract indicates that S. acuta has anticancer activity against A-431-human epidermoid carcinoma and HeLa-human cervical cancer cell lines. Conclusion: The present study concluded that the chloroform extract of S. acuta Burm.f. possess potent cytotoxic activity.