Application of DNA-based techniques for intraspecies differentiation of hake fish

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Abstract

Fish species identification is important as the interest of consumers in sea-fish meat is increasing. The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained on the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection.

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Hubálková, Z., Králík, P., Kasalová, J., & Renčová, E. (2009). Application of DNA-based techniques for intraspecies differentiation of hake fish. Acta Veterinaria Brno, 78(4), 673–678. https://doi.org/10.2754/avb200978040673

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