Colorimetric Determination of Maridomycin

Abstract

A new, rapid and sensitive method for the quantitative determination of maridomycin (MDM) is proposed. The assay method is based on the reaction of the epoxy group of maridomycin with picric acid in ethyl acetate. This reaction product has absorption maxima at 415 and 475~480 nm in alkali. The absorbance at 490 nm, in a region where the inter-ference from picric acid is negligible, follows Beer's Law up to 1000 μg of MDM per 1 ml of sample solution. This reaction is not interfered with by the presence of other 16-membered macrolide antibiotics. © 1979, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.