A kinetic and nuclear magnetic resonance study of methylated pyrimidine nucleosides

Abstract

Alkylated pyrimidine nucleosides are of interest from the viewpoint of mutagenesis and carcinogenesis. 1 H and 13 C nmr data are presented for a series of 2′-deoxynucleosides methylated at the O2-, O4-, and N3-positions of the base, and discussed in terms of their physical properties. The pH dependence of the stability of the O2- and O4-methylated 2′-deoxyribosides as well as the corresponding ribosides was examined by 1 H nmr and ultraviolet (uv) spectrophotometric methods.