Localization of Two Species Specific Antigenic Determinants on the Peptide Chains of Calf Skin Collagen

Abstract

Antibodies against two types of species specific determinants, designated P and S, can be differentiated in rabbit antisera against acid soluble calf skin collagen. Using the passive hemagglutination inhibition technique, they were localized with collagen α‐chains, with peptides obtained by treatment of the α‐chains with cyanogen bromide and with fragments isolated from collagenolytic digests of native collagen. The P‐determinant is labile to proteolytic attack (pronase, chymotrypsin, pepsin). It was detected in a 300 Å region at the C‐terminus of both types of α‐chains. The S‐determinant is resistant to proteases and was found to occur in an interior region of the α2‐chain. The serological activity is contained in one of the collagenolytic peptides. Copyright © 1970, Wiley Blackwell. All rights reserved