An NADP-dependent Glutamate Dehydrogenase in Chloroplasts from the Marine Green Alga Caulerpa simpliciuscula

Abstract

NADP-dependent glutamate dehydrogenase was partiafly purified from extacts of the marine siphonous green alga Caulrpa simplicius-cula. The enzyme had an apparent Km NH4+ of 0.4 to 0.7 mm and was highly specific for NADPH, a-ketoglutarate, and ammonium ions. The bulk of the NADP-gutamate dehydrogenase was isolated with the chloroplast fraction in cell-free preparations of this alga and was released from these "chloroplast fractions" as a soluble enzyme on gende lysis of chloroplast membranes.