Spectrophotometric determination of amiloride in bulk and pharmaceutical formulations

Abstract

Introduction: Simple, sensitive, rapid and accurate spectrophotometric methods have been developed for the determination of Amiloride in pure and pharmaceutical preparation. Methods: The method is based on the formation of ion-association complex of Amiloride with Wool fast blue(WFB) and Bromo cresol green(BCG) in acidic buffer followed by its extraction into organic solvent (chloroform). The absorbance of organic layer was measured at their respective wavelength of maximum absorbance against the reagent blank. The chemical reaction between Amiloride and dyes (WFB,BCG) have been studied by uv-visible spectrophotometric analysis. Results: The calibration curves were linear with correlation coefficient of 0.999 over the concentration range of 20-100 μg/mL for both the methods with maximum absorbance at 590 nm, 420 nm for WFB and BCG respectively. Different experimental parameters affecting the color development and stability of colored product are carefully studied and optimized. The results of analysis validated statistically and by recovery studies. Conclusion: The proposed methods are simple, rapid, accurate, precise and economical and can be used in the estimation of Amiloride in bulk and pharmaceutical formulations.