Total arsenic in tuna fish candidate reference material preparations: Homogeneity and stability testing

Abstract

Reference material plays an important role in analytical measurements. Total arsenic was analyzed in tuna fish samples using three different preparation methods for stability. The stability of total arsenic in tuna fish was evaluated over 12 months, under room temperature storage condition. Each measurement was conducted in triplicate in three different laboratories. Statistical analysis was carried out using Student's t-test. Two different methods were conducted to confirm homogeneity of the candidate reference material in this study. Random bottle number was applied in this analysis. Statistical analysis was carried out and the homogeneity for total arsenic was calculated using the one-way analysis of variance (ANOVA). The statistical results showed no significant changes in stability and the sample was homogeneous. The candidate reference material developed in this study demonstrated its suitability for quality assurance of the total arsenic measurement in tuna fish.