PHYTOCHEMICAL SCREENING AND BIOLOGICAL ACTIVITY OF ETHANOLIC EXTRACT OF ROSA X DAMASCENA MILL. CULTIVATED IN THE WESTERN REGION OF ROMANIA

Abstract

Rosa x damascena Mill. has pharmacological properties that are partially attributed to their abundance in phenolic compounds. We investigated the alcoholic extract from the plants petals. Microscopic examination of Rosa x damascena Mill. petals showed that both of the adaxial and abaxial epidermal cells contained pigments. The antimicrobial activity of the extract was determined by the disc diffusion method. The most sensitive antimicrobial effect was on the strain Pseudomonas aeruginosa. The SOD-like activity of Rosa damascena alcoholic extract has been tested both in vitro and in vivo. Three cell lines were used for determining the cytotoxicity of Rosa damascena alcoholic extract, a human cervical carcinoma line (HeLa), a human radical growth phase melanoma cell line (WM35) and a normal fibroblastic epithelial cell line (HFL1) by MTT method. The Rosa damascena extract presents SOD-like activity and is cytotoxic on HeLa and WM35 cell lines. Due to these results, Rosa x damascena extracts can be used for their bioactive potential.