A protocol for mass propagation through axillary bud proliferation was established for Rauwolfia serpentina L. Benth. (Apocynaceae). MS medium supplemented with 1.5 mg L-1 BA and 0.2 mg L-1 NAA elicited the maximum number of shoots (4 multiple shoots) from nodal explants. These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L-1 each of IBA and IAA. The in vitro raised plants were acclimatized in glass house and successfully transplanted to field condition with almost 95% survival. © 2008 Asian Network for Scientific Information.
CITATION STYLE
Salma, U., Rahman, M. S. M., Islam, S., Haque, N., Khatun, M., Jubair, T. A., & Paul, B. C. (2008). Mass propagation of Rauwolfia serpentina L. Benth. Pakistan Journal of Biological Sciences, 11(9), 1273–1277. https://doi.org/10.3923/pjbs.2008.1273.1277
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