Comparison of immunodiffusion and enzyme linked immunosorbent assay for antibodies to four Aspergillus species

Abstract

Antigenic extracts were prepared from Aspergillus fumigatus, A niger, A flavus and A terreus for use in enzyme linked immunosorbent assay (ELISA) and immunodiffusion (ID) tests for Aspergillus antibodies to determine whether the use of antigenic extracts from species other than A fumigatus increased the sensitivity of the ELISA. ELISA titres correlated well with positive ID tests. Patient titres by ELISA were significantly higher than control titres for all species. Patient titres to A niger were also significantly higher than titres to the other species. Total number of ID bands to A fumigatus correlated significantly with anti-Afumigatus ELISA titres. It is concluded that the use of antigenic extracts from species other than Afumigatus improves the sensitivity of the ELISA.