Protective role of curcumin against the toxic effects of cyclophosphamide in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9

Abstract

In the present study the effects of curcumin was studied against the toxic effects induced by 0.025 and 0.050 ml/ml of cyclophosphamide (CP) in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9 using hsp70 expression and dye exclusion test as a parameter. The exposure of the third instar larvae to 0.025ml/ml of CP along with 1, 5 and 10 mg/ml of curcumin results in the dose dependent significant decrease in the hsp70 expression and tissue damage for 12, 24 and 48hr of duration. Similar results were obtained with the exposure of third instar larvae to 0.050 ml/ml of CP along with 1, 5 and 10 mg/ml of curcumin. The selected doses of curcumin i.e. 1, 5 and 10 mg/ml were not toxic but reduced significantly the expression of hsp70 and tissue damage induced by CP. The results of the present study suggest that the curcumin has a protective role against the toxic effects of CP in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9.