Phytochemical analysis and antimicrobial properties of Psidium guajava leaves and bark extracts

Abstract

Objective:Psidium guajava is a plant used in traditional medicine for different ailments. The parts of the plant like leaves, fruit and the bark are used as medicine. They are used as antibacterial, antiparasitic, febrifuge, antispasmodic, to treat rheumatism, convulsions, and as astringent. The bark of this plant is used to treat dysentery, astringent and used as a bath to treat skin ailments. The objective of this study is to study the antimicrobial activity of Psidium guajava leaves and bark extracts and the bioactive compounds of leaves extracts by HPLC and NMR. Materials and methods: Psidium guajava leaves and bark were soxhlet extracted using different solvents. The antimicrobial activities were studied by agar well diffusion method. Phytochem. anal. was done and the methanol extract was subjected to HPLC. The purified Et acetate fraction was subjected to NMR. Results and conclusion: The leaves and bark extracts showed significant antibacterial and antifungal activity against Staphylococcus aureus, Streptococcus pyogenes, E.coli, Pseudomonas aeruginosa and Candida albicans. HPLC anal. of the leaf extracts revealed the presence of bioactive compound Quercetin.