Introduction: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. Methods: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. Results: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. Conclusions: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.
CITATION STYLE
Dos Santos, A. P., De Carvalho, M. E., Meirelles, L. R., & De Andrade Junior, H. F. (2018). Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys. Revista Da Sociedade Brasileira de Medicina Tropical, 51(5), 665–669. https://doi.org/10.1590/0037-8682-0391-2017
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