Efficiency of royal Jelly on in vitro fertilization in local Iraqi ewes

Abstract

This study was designed to investigate the effect of addition several concentrations of royal jelly to the media used in the in vitro maturation process of Iraqi sheep. The ovary was brought from the Al-Shulla slaughterhouse and transferred to the obstetric laboratory at the College of Veterinary Medicine at the University of Baghdad by a thermal insulating box containing the physiological solution with the antibiotic penicillin at a temperature of 30-38°C. In the laboratory, the ovaries were washed with a neutral buffer solution and then the oocytes were collected by two different methods slicing ovaryor follicle aspiration. The oocytes were classification into four groups depending on the layers of the cumulus cells surrounding the oocytes, as well as the homogenous of the cytoplasm. Oocytes that are completely or partially surrounded by cumulus cells and homogeneous cytoplasm considered normal oocytes but the oocytes denuded from the cumulus cells and heterogeneous cytoplasm considered abnormal oocytes was discarded. Normal oocytes were divided randomly into four groups at maturation; the first group was matured by TCM media alone and was considered as a control group.