Serum amyloid A as a marker of inflammation in xenotransplantation

Abstract

Increasing evidence indicates that inflammation plays a role in pig-to-baboon organ xenotransplantation. We have evaluated serum amyloid A (SAA) as a marker of inflammation in baboons with various pig xenografts. We measured SAA levels in recipient baboons with pig artery patch (n = 5), life-supporting kidney (n = 5), heterotopic heart (n = 2), or hepatocyte (n = 1) grafts and using an OmniChek-SAA for Inflammation & Infection kit. C-reactive protein (CRP), another marker of inflammation (e.g. D-dimer), was also measured.SAA indicated increased inflammation when baboons developed consumptive coagulopathy (CC; e.g. thrombocytopenia) or infection. SAA also indicated that treatment of the recipient with tocilizumab reduced inflammation. There was significant positive correlation between SAA with changes in CRP (r = 0.6, P < 0.05) and with D-dimer (r = 0.8, P < 0.01), but SAA appeared at times to more accurately reflect the clinical state of the baboon. In sum, measurement of SAA proved simple and quick, and indicated (1) significant inflammation when CC or infection was present, and (2) reduced inflammation when treatment with tocilizumab was administered.