M3 muscarinic receptor interaction with phospholipase C β3 determines its signaling efficiency

Abstract

Background: Scaffolding of signaling proteins to GPCRs may increase signaling efficiency and spatial fidelity. Results: PhospholipaseC(PLC) β3 binding directly toM3muscarinic receptor intracellular loops involves a non-canonicalPDZ interaction. Conclusion: M3 muscarinic receptor binding to PLCβ3 optimizes interactions with substrate and G protein activator. Significance: Scaffolding of PLC enzymes to GPCRs may be important for spatial signal specificity and efficacy. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.