Antimicrobial activities of laboratory produced essential oil solutions against five selected fungal strains

Abstract

It is well known that essential oils possess significant antimicrobial activity. This study was conducted to estimate the antimicrobial activity of various types of Biokill, a laboratory produced solution composed of several essential oils (Biokill dissolved in 96% ethanol; Biokill 96% further dissolved in DMSO; Biokill dissolved in 70% ethanol and Biokill 70% further dissolved in DMSO). The antimicrobial activity was evaluated against five selected fungal strains, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger I.N. 1110, Aspergillus sojae CCF and Penicillium spp. FNS FCC 266. A variation of the microtiter plate-based antimicrobial assay was used in order to assess the antimicrobial activity of the solutions. By applying this assay minimal inhibitory concentrations (MIC) of the Biokill solutions were determined for each strain of the selected test microorganisms. The results demonstrated that all variations of Biokill showed antimicrobial activity at concentrations lower than 2.5?g/mL. Biokill 70% further dissolved in DMSO showed the best antimicrobial properties against all the selected strains with MICs less than 1.25?g/mL. These results indicated that Biokill could find application in the pharmaceutical industry, in food preservation and conservation, in the prevention and treat?ment of plants infected by certain phytopathogens, etc.Dobro je poznato da esencijalna ulja poseduju znacajnu antimikrobnu aktivnost. Ovo istrazivanje je sprovedeno kako bi se procenila antimikrobna aktivnost razlicitih tipova Biokila, laboratorijski proizvedeni rastvor sacinjen od nekoliko esencijalnih ulja (Biokil rastvoren u 96% etanol; Biokil dalje rastvoren u DMSO; Biokil rastvoren u 70% etanol i Biokil 70% dalje rastvoren u DMSO). Antimikrobna aktivnost je ocenjena kroz pet odabranih gljivicnih sojeva, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger I.N. 1110, Aspergillus sojae CCF i Penicillium spp. FNS FCC 266. Varijacije na antimikrobni esej baziran na mikrotitarskoj ploci je bio koriscen kako bi se ocenila antimikrobna aktivnost na pomenuti rastvor. Ovim esejem je uradjena minimalna inhibitorska koncentracija (MIC) na Biokill rastvor za svaku vrstu/soj odabranih test mikroorganizama. Rezultati su pokazali da sve varijacije na Biokil poseduju antimikrobnu aktivnost pri koncentraciji nizoj od 2.5 ?g/mL. Biokil 70% dalje rastvoren u DMSO pokazao je najbolja antimikrobna svojstva za sve odabrane sojeve i MIC niza od 1.25 ?g/mL. Ovi rezultati pokazuju da bi Biokil mogao da nadje primenu u farmaceutskoj industriji, u prezervaciji i konzervaciji hrane, u zastiti i tretmanu biljaka koje su inficirane odredjenim fitopatogenima itd.