In Vitro Propagation of Paradox Walnut Rootstock

Abstract

Apical and lateral meristems of Paradox walnut ( Juglans hindsii x J. regia ) were used to investigate the possibility of accomplishing in vitro propagation of Paradox rootstock. A walnut specific medium, DKW, has been defined supporting optimum multiple shoot development under 4.5 μm benzyladenine (BA) and 5 nm indolebutyric acid (IBA) treatment. The method of analysis to determine the optimum level of constituents in tissue culture medium is discussed. Also reported is the in vivo rooting of tissue culture derived shoots.