Isolation and Biochemical Characterization of Extracellular Proteolityc Enzym from Lactic Acid Bacteria Isolated from Various Indonesian Traditional Fermented Product (Bakasam)

Abstract

Local isolates of lactic acid bacteria isolated from Bakasam had potential to producing extracellular proteases. The research was carried out with the purpose of knowing the biochemical character of protease produced from isolates of lactic acid bacteria. The results showed extracellular protease isolates BAL 2 (Lactobacillus acidophillus) has a specific activity i.e. of 0.89 U/mg. Biochemical characterization of proteases indicates optimum pH 5.5, and is inhibited by chelating agent EDTA. The result of the purification with Sephadex G-100 column showed a band on SDS-PAGE with the size of molecular weight approximately 90 kD.