Quantifying anti-HBV effect of targeted ribonuclease by real-time fluorescent PCR

Abstract

Aim: To quantify the inhibition of HBV replication by targeted ribonuclease by using real-rime fluorescent PCR. Methods: Targeted ribonuclease was introduced into 2.2.15 cells by liposome-mediated transfection or HIV-TAT mediated protein transduction, Forty-eight hours after the transfection and 24 h after the transduction, supernatants of 2.2.15 cells were collected and HBV DNA in the supernatants was quantified by real-time fluorescent PCR with a commercial kit. Results: HBV DNA concentrations in the supernatants of 2.2.15 cells transfected or transducted with targeted ribonuclease were 4.9±2.4×108 copies/L and 8.3±4.0×108 copies/L, respectively. Compared with controls, transfection or transduction of targeted ribonuclease reduced HBV DNA concentration in the supernatants of 2.2.15 cells by 90.4% and 90.1%, respectively (P<0.05). Conclusion: Targeted ribonuclease can inhibit HBV replication in 2.2.15 cells. Copyright © 2004 by The WJG Press.