Enhancing Alfalfa Productivity with Tuber: Associated and Cellulolytic Bacteria in the Aral Sea Region

Abstract

Enhancement of soil fertility and yield via application of microbial preparations to increase alfalfa productivity presents a potential sustainable approach to mitigate famine through augmentation of protein-rich food supplies. This study investigates the influence of active strains of tuber-associated and cellulolytic bacteria on alfalfa productivity using traditional microbiological and agronomical methodologies. Varied strains of Sinorhizobium meliloti bacteria, in combination with cellulolytic bacteria and mineral fertilizers, were tested on alfalfa plants. Multiple replicates for each treatment were conducted, inclusive of a control group without bacterial application. A significant increase in the germination rate of alfalfa seeds, ranging between 80-90%, was observed following the application of microbial preparations based on cellulolytic bacteria. Variant 4 W (CLB+ Sinorhizobium meliloti IMVZH5-1) exhibited a higher density of plants per square meter (1205 pieces). When employing this preparation, the amount of green matter was 47 C/ha, the production of alfalfa was 3 C/ha higher, and the seedling absorption rate was 11.1% higher compared to the control group. The biggest contribution to the rise in the number of inorganic nitrogen substances in the soil came from the application of CLB and ANP fertilizers. The results obtained can be used as a basis to increase the alfalfa yield in the conditions of the Kyzylorda region.