Development of an Ultra-SensitiveEnzyme Immunoassay for Insulin and Its Application to the Evaluation of Diabetic Risk by Analysis of Morning Urine

Abstract

Currently available enzyme immunoassay methods for peptides can be divided into two groups, homogeneous and heterogeneous methods. Homogeneous enzyme immunoassay methods, in which signals that are directly obtained from a mixture of test samples and reagents correlate with the amount of peptide in test samples, are simpler and quicker but less sensitive than heterogeneous enzyme immunoassay methods, in which free and bound forms of enzyme-labeled reactants are separated from each other.