Morphological and Simple Sequence Analysis of Tangerine (Citrus nobilis L.) from Three Regencies in North Sumatra

Abstract

Tangerine or Mandarin orange (Citrus nobilis L) is a well known citrus in the world and becomes a fruit commodity in Indonesia. Various types of local tangerine have been produced with different name depends on the village and Regency where the citrus are grown. However, the information about the genetic variation of the citrus are limited. The research is aimed to study the genetic similarity of the local tangerines by using morphological and simple sequence analysis. The research was carried out by collecting citrus samples from eight villages that are spread in three Regencies at North-Sumatera. The morphological characteristic of the citrus are documented, and the DNA are analyze by using simple sequence repeat (SSR) for their genetic variation. The results have revealed that different tangerines (local named as Brastepu, Maga, Sipirok) are having similar morphological characters. The protein in the DNA are containing 48 bands (100 bp-300 bp), consisted of 30 polymorphic bands and 18 monomorphic bands, and have compared to four DNA primers. Analysis of the genetic diversity by using NTsys software found that they are clustered on 0.74 similarity coefficient value and the local citrus are devided into 3 groups. The lowest genetic distance on the Sibanggor Tonga with Baringin Siumuran was 0.63 (63%), meanwhile the highest distance was 1.0 (100%) on Huta Namale with Huta Lombang, Aek Kambiri, and Aek Horsik..